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To the best of our knowledge, this is the most comprehensive model thus far and also the first to explicitly comprise compartmentalization. What is important, we have managed to achieve balance between the complexity and biological soundness of the model and its computational tractability.
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This periplasmic compartmentalization strategy comprises a number of compelling advantages, in particular with respect to artificial metalloenzymes, which lead to a drastic increase in the throughput of screening campaigns and additionally are of unique value for future in vivo applications.
Using this model, we demonstrate that compartmentalization of the enzymes comprising an engineered pathway for biosynthesis of R-1,2-propanediol leads to improved kinetic properties for the pathway enzymes, especially when substrate diffusivities are low.
However, the intermingling of these clades with clades comprised of both genital and blood sequences and the absence of tissue-specific genetic features suggests compartmentalization between blood and genital tract may be due to viral replication and proliferation of infected cells, and questions whether HIV-1 in the female genital tract is distinct from blood.
Focus on compartmentalization.
Compartmentalization of digestion.
Martin, W. Evolutionary origins of metabolic compartmentalization in eukaryotes.
But in recent decades such compartmentalization has disintegrated.
Caschera, F. & Noireaux, V. Compartmentalization of an all-E.
Perpetuate the artificial compartmentalization of knowledge by field.
Compartmentalization is an important organizational feature of life.
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Since I tried Ludwig back in 2017, I have been constantly using it in both editing and translation. Ever since, I suggest it to my translators at ProSciEditing.

Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com