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A comprehensive random PB2 C-terminal truncation library was constructed using an almost identical approach, but differing in cleavage of pYAN009 with NsiI and NotI located at the 3' end of the PB2 gene insert that had been cloned in frame with a 5' sequence encoding N-terminal TEV-cleavable hexahistidine tag.
Several previous studies have provided sets of essential genes of organisms predicted from comprehensive random mutagenesis or targeted gene disruption.
Beside IS 6100 quite a few active mobile elements have been published for C. glutamicum which are of restricted usability of generating a comprehensive random transposon library since they prefer specific sequences, e.g. a triple A or T (IS 1249) [ 20] or palindromic sequences (e.g. IS 14999) [ 23], as a target for integration.
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"But we have a comprehensive random-testing program for steroids and other performance-enhancing drugs".
There was no difference between comprehensive and random estimates of SC CSA.
The North says it never has — a denial Washington wants to verify through comprehensive inspections, including random visits and the taking of samples in all sites suspected of being involved in nuclear activities, including some undeclared military sites.
The sample constituted by these four sites is neither a comprehensive nor a random sample of BitTorrent sites.
Although the ROM database represents neither a comprehensive nor a random sample of Algonquin Park lakes, there is no reason to think that the sampling should bias the presence or absence of hybrids or parental species among lakes in the database.
The comprehensive, subjective and random mean CSAs are provided in table 1. Subjective estimates of SC CSA were significantly higher than the comprehensive mean (table 1).
Nevertheless, this technique relies on the comprehensive sequencing of random fragments of RNA with little attention to transcription start sites (TSSs) [ 28].
After that, Kendall [3] and Matheron [4] provided a comprehensive mathematical theory of random sets which was greatly influenced by the geometric probability prospective.
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