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Five compounds were subsequently selected for experimental assay.
These bioactive compounds were subsequently tested in a tertiary polyglutamine aggregation assay, which identified five inhibitors.
These compounds were subsequently shown to enhance the uptake of doxorubicin by MCF-7 cells that over-expressed Pgp.
The hit compounds were subsequently subjected to filtering by docking and visualization, to identify the potential lead molecules.
The 35Cl Nuclear Quadrupole Magnetic Resonance (NQR) spectra of these compounds were subsequently measured over a temperature range from approximately 100 K to 300 K [2].
These two compounds were subsequently evaluated for in vivo anticancer activity against the growth of a human advanced stage SC UACC-257 melanoma in skid mice.
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After 12 h, 10 μmol/L of compounds was subsequently added and incubated for 48 h.
The potency of the isolated compounds was subsequently investigated by in vitro kinase assays.
These compounds are subsequently digested into glucose by brush border enzymes of the duodenum and jejunum.
The purified compound was subsequently subjected to 1H and 13C NMR spectroscopic analysis.
The latter compound was subsequently deprotonated and used to polymerize ethylene oxide by a classical core-first approach.
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