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Compounds were loaded into gels in TBE urea buffer (7:20 compound:loading buffer).
Compounds were loaded into a JChem database [23].
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Aliquots (10 μg) of each isolated compound were loaded on five aluminium-backed thin layer chromatography (TLC) plates (Merck, silica gel 60 F254) and were prepared in the solvent systems mentioned above.
To facilitate dereplication, or the identification of known compounds in subsequent work, aliquots (10 μl) of 1 mg/ml solutions in chloroform (CHCl3) (equivalent to 10 μg) of each isolated compound were loaded on each of three aluminium-backed thin layer chromatography (TLC) plates (Silica gel 60 F254, Merck) and eluted in three mobile systems of differing polarity [ 12].
Methylene blue as a model active compound was loaded on the fiber mats.
Moreover, the synthesized BPP compound was loaded in to the PHB-PCL nanofibrous scaffold to check the cytotoxicity against the MCF-7, Hep-2 and NIH 3T3 fibroblast cell lines.
Cell plates and compound plates were loaded into a Hamamatsu FDSS 6000 kinetic imaging plate reader.
Compounds to be docked were loaded as.mol files into Discovery Studio Visualizer where hydrogens and relevant charges were added before the structures were minimised to relax bond lengths and fix bond angles.
RAW264.7 murine macrophage cells were loaded with compound 3 and then treated with 100 μM hydrogen peroxide for 5 min to show the fluorescence increase.
Compounds of interest (Oligomycin and FCCP) were loaded in the drug delivery system of sensor cartridge before calibration.
During these 60 min, the ports of the cartridge containing the oxygen probes were loaded with the compounds to be injected during the assay (50 μl/port) and the cartridge was calibrated.
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