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The compounds were initially profiled in a panel of 24 cancer-relevant protein kinases.
The compounds were initially docked into the binding pocket of the homology model of 5-HT3 receptor using GOLD program.
Full details of all compounds tested can be found in Supplementary Table 2. Compounds were initially formulated in dimethyl sulfoxide (DMSO) as a 1000x stock solution (w/v) as previously described24.
In the present study, a number of compounds were initially designed and manufactured, based on a previously studied and manufactured isolator compound.
53 compounds were initially identified to modulate the activity of ALDH2 by a high-throughput esterase screen from a library of 63,000 compounds.
These compounds were initially evaluated for histamine H3 receptor binding affinities, suggesting that a propoxy chain linker between the amine and the core ring could be essential for optimal binding affinity.
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We also examined the effect of DMSO on this optimized enzymatic reaction of 3MST, because the library compounds are initially dissolved in DMSO as stock solutions.
Processing of hydrophobic organic compounds into nanoparticle form by rapid precipitation requires the use of organic solvents in which the compounds are initially dissolved.
Further, many compounds are initially legal due to the regulatory challenge of trying to outlaw the large numbers of possible drug analogs that may be synthesized and distributed; authorities throughout the world have struggled with this issue [11, 12].
In HTS, millions of compounds are initially screened to assess biological activity against a target.
The library of compounds is initially selected based on docking to the antiparallel β-sheet of Aβ16 21.
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