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Compounds were arrayed into 384-well microtiter master plates at a concentration of 10 mM in DMSO.
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White colonies were arrayed into 384-well plates for sequencing.
All compounds were docked into 15-LO.
Compound docking: All compounds were docked into an unliganded structure of mouse Δ IBB-importin-α1.
Compounds were loaded into gels in TBE urea buffer (7:20 compound:loading buffer).
Compounds were grouped into 32 clusters and 8 singletons.
100 μl of compounds were added into wells.
Compounds were separated into appropriate aliquots and stored at −20°C.
All biomass compounds were placed into the try-set.
Samples were manually arrayed into three 96-well master plates.
All qualifying compounds were further organized into target sets.
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