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Where appropriate, compounds were added to both the lower and upper chambers.
Compounds were added at different dilutions and kept for 18 hrs.
Cells were washed with HBSS twice, and recording buffer (2.5 mM probenecid in HBSS) and various compounds were added.
Various compounds were added to each buffer based upon the experiments being performed (K4PPi, Na4PPi, K2HPO4, Na2HPO4, IDP, etidronate).
Serial dilutions of indicated compounds were added and incubated for additional 72 h.
After 12 h, compounds were added and incubated for further 24 h.
Small chemical compounds were added into KSR medium 1 day after reseeding of virus infected MEFs into 96-well plate.
All of the compounds were added at a level to achieve an initial headspace concentration of 2000 ppbv.
Compounds were added to the RD cells in a concentration series from high to low and infected by the virus.
Various concentrations of compounds were added to obtain final concentrations of 0.5, 5, 50 μg/mL, each in triplicate.
As the interfering heavy metal ions and aromatic compounds were added, there was much less change in the current.
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