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The potential of creating computer-assisted methods for the structure elucidation of new organic compounds was first discussed in the second half of the past century.
Eugenol, a natural substance used as a target molecule for the manufacture of bioactive compounds, was first isolated in 1929 and its commercial production began in 1940 in the United States.
The carcinogenic role of arsenic compounds was first noted over 100 years ago in the Hutchinson (1887) observation that an unusual number of skin tumors developed in patients treated with arsenicals.
This widely shared view about the constant proportions of elements in compounds was first explicitly proclaimed as the law of constant proportions by Joseph Louis Proust (1754 1826) in the first years of the 19th century.
The ability of a xenobiotic substance to induce protection against subsequent exposure and also to induce protection against exposure to other potentially toxic compounds was first described more than 30 years ago by Hans Selye (1971).
The estrogenic activity of these compounds was first discovered in the 1940s, when they were indicated as the cause of infertility of Australian sheep who grazed on clover (Trifolium subterraneum) whose leaves contain high amounts of the isoflavons biochanin A and formononetin (Cornwell et al., 2004).
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All the synthesized compounds are first reported.
The compounds were first tested in an HIV cell-cell fusion assay with the most promising evaluated in a second, more biologically relevant viral entry assay.
Thus, various sodium-based compounds were first investigated and three candidate substances satisfying several criteria were selected; sodium bromate (NaBrO3), sodium chlorate (NaClO3), and sodium tetrafluoroborate (NaBF4).
The in vitro cytotoxic activities of target compounds were first investigated against two human cancer cell lines including Eca109 and Huh7 by MTT assay.
All the compounds were first inspected for anti-proliferative activity using ER-α responsive T47D breast cancer cell lines and ER-α binding assay.
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Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com