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The ability of the three compounds to stimulate NO release was completely blunted in the aortic endothelial cells extracted from ERα KO mice (Figure 2D) or in aortic endothelial cells taken from ERα WT mice and Swiss mice in the presence of Fulvestrant (Figure 2C and 2E).
This is despite the well-known effect of arsenic compounds to stimulate the formation of stress granules and P (processing) bodies, both thought to be involved in localizing mRNA decay (Buchan 2014).
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However, just monitoring the ability of a compound to stimulate uterine growth is not sufficient to characterize its estrogenicity.
This outcome was not entirely unexpected because the antagonist-mode assays all required the pre-addition of nonsaturating levels of an agonist compound to stimulate the receptor signal before test compounds could be screened, which introduces an extra source of variance.
Differentiated L6 myotubes were treated with 5-aminoimidazole-4-carboxamide-1-β-4-ribofuranoside (AICAR) and/or compound C to stimulate and/or block AMPK respectively.
To assess coupling of hFFA2-C4.57G/H6.55Q to Gαq/11 pathways, the ability of C3 and compound 25 to stimulate Ca2+ mobilization was examined in cells expressing wild-type hFFA2 (Fig. 9 C) or hFFA2-C4.57G/H6.55Q (Fig. 9 D).
The presence of luxS in many phylogenetic groups within the domain Bacteria indicates that these bacteria, while recycling SAH in a two-step enzymatic process, at the same time produce a compound able to stimulate luminescence in a V. harveyi reporter strain which is most probably a furanosyl-borate-diester.
Although several compounds are reported to stimulate C2C12 cell differentiation in vitro, there has been no systematic analysis of compounds promoting C2C12 differentiation in the presence of TGF-β1.
Indeed, Bis 31/31')[Cys31, Nva34]NPY(27-36)-NH2, presenting a high sequence and scaffold homology with our compounds was able to stimulate in vivo the food intake in rats in a similar manner than that observed by other non-dimeric Y5 selective agonists [37].
These compounds were able to stimulate the production of βH crystals in the absence of any biological sample.
These compounds are able to stimulate CD4+ T lymphocytes that represent the major source of the IL-2 cytokine [ 66].
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