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But when fetal bovine serum was removed or cells were chemically induced with specific compounds, the expression of these proteins became high.
To investigate whether IAA biosynthesis is involved in induction of T3SS by the phenolic compounds, the expression of hrpN in the wild-type Ech3937 and Ech138 was compared with the addition of OCA.
In parallel with the increased uptake of the above radioactively labelled compounds, the expression of the FBP gene in KB-CP1 cells was also increased after treatment with DAC for 3 days and then 3 days in DAC-free medium as detected by RT-PCR.
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The effect of local inflammation on this pathway would similarly compound the expression of RANKL.
The inhibitory activities of synthesized compounds against the expression of miR-21 were evaluated using stem loop RT-qPCR and compound 1j was discovered as the most potent compound, which displayed a time and concentration dependent inhibition manner.
It was observed that all tested metal compounds induced the expression of HSP90 gene; however induction pattern was different according to the tested metal compound.
Most compounds inhibited the expression of viral antigens HBsAg or HBeAg at low concentration.
Zebrafish are also proving key to advancing our understanding of how particular chemical compounds affect the expression of individual genes that maintain and influence virtually every body system.
To test whether these compounds modify the expression of specific genes, we analyzed the effect on the gene expression in AZ521 cells by differential display analysis using the compounds showing different characteristics in the recognition of specific DNA sequence.
RT-PCR and Western blotting experiments further supported that both compounds altered the expression of mRNA and receptor protein of ER-α, thereby preventing the further transactivation and signalling pathway in T47D cells lines.
Objective To determine the effects of recombinant equine IL-1β and a number of antiinflammatory compounds on the expression and activity of inducible nitric oxide synthase (iNOS) in cultured equine chondrocytes.
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