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The yellow color of the nitro compounds solution has not changed which confirmed the catalytic role of nanoparticles.
A 20 µL of acarbose or test compounds solution was incubated for 7 min with 150 µL of enzyme stock at 37 °C.
These containing phosphate buffer 118 μL(0.1 M, pH 8.0), DTNB 6 μL(4 mg/mL for AChE or 8 mg/mL for BuChE), different concentration of tested compounds solution 15 μL and AChE or BuChE solution 5 μL, were mixed and incubated for 15 min at 37°C.
ADS-I and its metabolites (M1, M2) were dissolved in DMSO (<1 ‰) and diluted in DMEM, A volume of 100 µL compounds solution were added to each well and made the final concentrations were 5, 10, 20, 50, 100 µM, respectively.
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The compound solution (100 μg/mL) was smeared on the whole plant.
Each compound solution in acetone was then suspended in distilled water with Tween-80 (0.001%).
The microstructures of corroded steel bar in seawater and compound solution were observed by SEM.
C, uncoated catheter; C + N, catheter coated with nanoparticles; 1a, 1b, catheters immersed in the compound solution; 1a + N, 1b + N, catheters coated with N and the chemical compound solution.
12 h later, the compound solution (100 μg/mL) was smeared on the whole fully open leaves.
To determine 100%% PP-1 activity, 10 μL of 50%% (v/v) aqueous methanol was used instead of compound solution.
The virus was inhibited by mixing it with the compound solution at the same volume for 30 min.
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