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The concentrations of compounds required to inhibit the growth of these tumor cells by 50% is in the range of 10−8 M.
The IC50 (the concentration of test compounds required to kill 50% of cell population) was determined (Table 1, Fig. 3).
Data generated were used to plot a dose response curve of which the concentration of test compounds required to kill 50% of cell population (IC50) was determined.
Data generated were used to plot a dose response curve of which the concentration (μM) of test compounds required to kill 50% of the cell population (IC50) was determined.
Data generated were used to plot a dose response curve of which the concentration of test compounds required to kill 50% of cell population (IC50) was determined (Fig. 3).
The data generated were used to plot a dose-response curve of which the concentration of test compounds required to kill 50% of the cell population (IC50) was determined.
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50% cytotoxic concentration (CC50) values represent the concentration of compound required to kill 50% of the fibroblast cells.
Another metric for evaluating antimicrobial activity, the MBC, determines the concentration of antimicrobial compound required to completely kill bacteria (rather than just inhibiting growth).
Inhibitory concentration 50% (IC50) in μM is defined as the concentration of experimental compound required to inhibit 50% of the conversion of MTS to formazan, as compared with the absorbance produced by untreated cells after 16 h of incubation.
Table 3 CC50 values and selectivity index (SI) of the most active compounds on normal VERO Test compound (CC50)a (µg/mL) Selectivity index (SI b 10 250 10 13 125 5 aCC50 is the concentration of compound required to kill 50% of the fibroblast cells bThe selectivity index (SI) was calculated using the formula, SI = CC50/MIC.
Using the DPPH free radical assay, according to a modification of our previously reported procedure [43], the radical scavenging activity of this new series of thiazolyl coumarins 10a-h was determined and expressed as IC50 values [41, 43], which is the concentration of tested compound required to scavenge 50% of the DPPH radical concentration (0.11 mM in this case on dilution).
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