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Khat contains the active compounds of cathinone and cathine.
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The elution program (2) used for the analysis of cathinone derivatives and other compounds was as follows: 5 % B to 20 % B (0 20 min), and up to 80%% B (20 30 min, 10-min hold).
All the samples were immediately frozen (−20 °C) to prevent decomposition of cathinone.
The purity of cathinone, cathine and norephedrine, isolated by preparative HPLC, was determined by analytical HPLC DAD reported by [13].
The oxalate salt obtained from the procedure described above was found to contain a mixture of cathinone, cathine and norephedrine.
After extraction, SPE extracted plasma with cathinone and mephedrone samples were either analyzed directly by LC-MS/MS or first spiked with known concentrations (0 250 ng/ml) of cathinone and mephedrone as "standard addition" samples.
This study is aimed to use the standard addition method to overcome the plasma matrix effect on the quantification of cathinone and mephedrone by LC-MS/MS.
Though the ingredients can vary, bath salts normally contain some type of cathinone stimulant like mephadrone.
This transformation is a well-known characteristic of cathinone derivatives.
The contents of the NRG products have varied substantially from one type of cathinone, to a combination of cathinones, to banned piperazines, and to inorganic material or benzocaine.
Khat chewing produces psychostimulation effect in the form of euphoria and excitement because of cathinone contents [ 5– 7].
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