The NITEGE 681-3/NEP522 sandwich ELISA has applications for screening compounds for aggrecanase(s) inhibitory activity, selection of appropriate OA models, the evaluation of compound efficacy in vivo, as well as the potential to stratify patients for clinical trial design.
The replicon system enables cell-culture compound efficacy evaluation against an active replication complex, and a functional replicon dependent upon a genotype 2b polymerase would augment existing cell-culture efficacy studies that are presently limited to genotype 1a, 1b, and 2a replicons.
Compound efficacy has been observed also in ICD mouse model.
Histology and immunohistochemistry confirmed compound efficacy at 0.5 and 1 mg/kg in modulation of innate and adaptive immunity in mouse models of contact skin reaction.
Indeed, a great advantage of chemical screens in zebrafish is the ability to rapidly assess compound efficacy and toxicity in vivo.
To assay compound efficacy, synchronized pha-1 Htn-Q150 pha-1 Htn-Q150 pha-1 Htn-Q150nd without drug for seven days, then scored for degeneration using the dye-fiL1ing assay (Figure 3A).
C. elegans N2[wild-type] and GE24[pha-1 e2123)], E. coli food sources OP50 and HB101 (OP50 for compound efficacy and aging assays and HB101 for sensitized and food clearance assays) were obtained from the Caenorhabditis elegans Genetics Center.
An in vitro TTR amyloid fibril formation assay was utilized to evaluate compound efficacy and the three-dimensional crystal structures of TTR in complex with four inhibitors were solved.
a Maximal reference compound (efficacy = 1.0) ; efficacy of other compounds are relative to the maximal reference compound.
