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An integrated fixed-film activated sludge (IFAS) system is one of the most popular modified activated sludge processes which increases the microbial population and accelerates the biodegradation of organic compounds by adding a fixed media to a suspended growth basin (Kim et al. 2010).
Standard curves were generated for quantitation of the CoA compounds by adding known amounts to cell extracts (data not shown).
Cell proliferation was assayed at 48 72 h after treatment of compounds by adding 20 μl of 5 mg/ml MTT solution per 100 μl of growth medium.
The next stage in this strategy is to optimise these fragments to more potent compounds, by adding substituents which make further binding interactions with the enzyme active site.
The steroid monooxygenase and the P450 may participate in detoxification of LPPE compounds by adding or altering functions of metabolites, similar for example to the P450s involved in fungal detoxification of pisatin [ 44].
Compound screening, that is, a screening to assess the physiological and possible therapeutic effects of chemical compounds by adding them to the water in the ZF tank, can be done using different assays and via a targeted or a blind approach.
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Our errors are often compounded by adding other recipients to the 'cc' list and, even worse, the 'bcc' list.
"Aren't you just compounding things by adding another to the mix?" Mr. Deveny asked, playing the devil's advocate.
But, Ms. Moorthy asks, if the name was a mistake, why compound it by adding "fast track" as the location?
We report here the synthesis and validation of azi-isoflurane, a compound constructed by adding a diazirinyl moiety to the methyl carbon of the commonly used general anesthetic isoflurane.
Total phenolic compounds were assayed by adding 400 mL sample extract into two 15-mL tubes containing 600 mL 80% (v/v) methanol, 5 mL 10% (v/v) F-C reagent, and either 4 mL saturated Na2CO3 (75 g L−1) or 4 mL water.
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