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Interference with MT polymerization was validated by the compounds' ability to inhibit MT assembly at the biochemical and cellular level.
The researchers tested the new compounds' ability to reactivate viral reservoirs in J-Lat cell lines, which contain latent HIV and begin to fluoresce when they express the virus.
Zadina's team tested the compounds' ability to dull pain by injecting them into the brains and spinal cords of mice, then placing each mouse's tail under a hot light.
The controversy: A 2005 study found that constant application of antiperspirants containing aluminum chloride or aluminum chlorhydrate could be related to an increase in breast cancer cells because of the compounds' ability to mimic estrogen in the body.
We speculate that these observations may result from the compounds' ability to enter the cell.
In addition, a shortcoming of these assays is that they do not assess for a compounds' ability to modulate chemosensitivity.
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We conducted a secondary screening to evaluate these compounds' abilities to increase TJ permeability.
As TJ opening by capsaicin is associated with Ca2+ influx, we first screened the compounds that induce Ca2+ influx in layered MDCK II cells, and then we determined the compounds' abilities to open TJs.
Unfortunately, there was barely microorganism possessing the compound ability of lignocellulose decomposition and solvents production natively.
In separate experiments, CellEvent Caspase 3/7 reagent (Life Technologies) was used to evaluate compound ability to trigger apoptosis signaling.
The main component of stone fish tissue extract with the highest peak area of 59.7% identified by GC/MS was dimethyl sulfoxide, which is an amphiphilic compound with ability to dissolve lipophilic compounds [ 22].
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