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In order to improve the compound-target ratio, targets with only one compound were removed from the database resulting in a dataset of 597 compounds covering 91 targets.
Next, all compounds whose distance was >3 standard deviations from the strongest activating compound were removed to reduce outliers.
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Besides, when the SS mesh was placed into the CVD reactor and the temperature was increased to 300 °C, the nitrate compound was removed.
The compound was removed by rinsing three times with FAS.
When the compound was removed after 48 hours of incubation, frataxin protein levels rapidly increased up to ∼6 fold over baseline at 6 hours after the drug washout, and this marked increase was still present at 24 hours (Figure 2G).
Under such circumstances, we compared basal autophagy in a time course after the LY294002 compound was removed and substituted with growth medium (Basal) or amino acid-free medium (EBSS) with or without protease inhibitors (Fig. 5B, 5C).
Excess OCT compound was removed by centrifugation in PBS.
This compound was removed by partition in the aqueous fraction (see Figure 1).
OCT compound was removed from cryosections by washing for 15 min in phosphate-buffered saline (PBS) at 37°C.
After an additional exposure period, the second compound was removed and cells were allowed to recover in drug-free medium for 72 h.
The O.C.T. Compound was removed from the slide by incubating the slide in a Petri dish containing deionized water, until the media was solubilized, and the sections were detached from the microscope slide.
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