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Moreover, spectroscopic characterization and cellular localization for selected compound were performed.
Single concentration measurements at 10 μM compound were performed using ALDH1A1, ALDH1A2, ALDH1A3, ALDH2, ALDH1B1, ALDH3A1, ALDH4A1, and/or ALDH5A1 to determine the selectivity of these 12 compounds toward ALDH2.
From the optimized geometry of the molecule, vibrational frequencies, gauge-independent atomic orbital (GIAO) 1H and 13C NMR chemical shift values, molecular electrostatic potential (MEP) distribution, non-linear optical properties and frontier molecular orbitals (FMOs) of the title compound were performed at B3LYP/6-311G d,p).
Calibration curves for each compound were performed by plotting the peak area (y) against the concentrations (x, μg/ml).
All the quantum chemical calculations of the studied compound were performed by applying DFT method with the B3LYP functional and 6 311G (d,p) basis set using Gaussian 03 software [26].
Two experiments using different delivery protocols of the compound were performed.
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Further structure elaboration of a representative compound was performed via Pd catalyzed C C bond forming reactions.
In vitro anti-diabetic activity of synthesized compound was performed by SLM model.
The TGA of the studied compound is performed over the temperature range 25 800 °C under flowing nitrogen atmosphere and the result is shown in Fig. 11.
Retention time of 4-ketopinoresinol was 145 mm and quantification of the compound was performed by measuring their peak areas as described above.
Blocking experiment (10 mg/kg cold compound) was performed at 30 min time point in healthy female adult mice n=3 and compared with vehicle treated batch.
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