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TACs of the dynamic scan presented in Figure 2 (right part) also depict the result of a displacement experiment in which 10 mg/kg of unlabeled compound were injected at 82 min post-injection.
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The test compound is injected intraperitoneally prior to carrageenan injection while using a standard commercial fibrinolytic agent as positive control and saline as negative control (Yan et al. 2009).
When a dose of the new compound is injected, most of it washes out quickly.
In another series of experiments, the compound was injected into young mice before their hair fell out.
The test compound is injected prior to the thrombus formation.
Novartis' compound is injected every few months and could hit the market in a few years.
The test compound is injected intraperitoneally prior to the application of ferric chloride.
Then, the bulb was evacuated using a vacuum pump and approximately 1 μL of pure compound was injected through a rubber septum.
Background-subtracted CVs were calculated by subtracting the average of 10 background scans, taken before the compound was injected, from the average of five CVs recorded after the analyte bolus was injected.
At time = 0 s the compound is injected on the protein surface and rapidly binds the immobilised protein, interaction is allowed to take place for 30 s, after which the injection stops and the compounds dissociate from the surface.
To see whether the GHS-R1a antagonist, d-lys-GHRP-6, was able to interfere with the central anti-hyperalgesic and anti-inflammatory effect of ghrelin, the compound was injected i.c.v. 5 min before ghrelin (1 nmol/rat, i.c.v).
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