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The solid deposits formed from the model compound were analyzed by TEM to determine their internal structure.
The carbon and hydrogen content in the intercalation compound were analyzed on a CHNS-932 (LECO Instruments, Michigan, USA).
Samples coated with a matrix compound were analyzed by a MALDI LTQ Orbitrap XL mass spectrometer at a resolving power of 30 μm, spatial resolution.
Data from the competition plots (as arithmetic means of values derived from three different assays, each in triplicate for each compound) were analyzed and subsequently IC50 and K i values were calculated using GraphPad Prism® software (San Diego, USA).
The complete vibrational studies of the compound were analyzed by FT-IR, FT-Raman and UV visible spectra combined with Normal Coordinate Analysis (NCA) following the scaled quantum mechanical force field methodology and density functional theory (DFT).
To proof this prediction, HaCaT cells treated with each compound were analyzed for the induction of pro-inflammatory cytokines (IL1-β, TNF-α, IL-8 and IL-6).
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Tetramethylsilane is an inert liquid added in small amounts to the compound being analyzed.
Moreover, supramolecular assembly of the title compound was analyzed by density functional theory (DFT) calculations.
In further steps each compound was analyzed for its possible application as a drug.
In this study, each compound was analyzed by enumerating their distinct targets over their demonstrated biological activity.
The chemical structure of each enriched bioactive compound was analyzed by nuclear magnetic resonance and mass spectroscopy.
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