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Aliquots (about 100 μL) of each individual pure compound were added to a series of 4-mL glass vials, and the following procedures for the vaporization experiment were identical to those of the first series.
For polymersomes encapsulating 1,1'-Dioctadecyl-3,3,3',3'-tetramethylindodicarbocyanine perchlorate (Sigma) 10 µg of this compound were added to the polymer solution.
Fresh media and compound were added every 24 h.
Various concentrations of AZ-1 compound were added and incubated at 37°C.
Signals of all detected charge states and isomers corresponding to the same compound were added up.
To prevent effects from other unknown kinases in the lysate, 20 μM PKC inhibitor peptide, 2 μM PKA inhibitor peptide, and 20 mM R24571 compound were added to the kinase reaction buffer.
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But after a chemical compound is added — in this case, water — the nanofibers become unglued and the material becomes soft.
2.5 g of the ammonium compound was added to the mix.
Hristov authored a previous study that showed methane emissions dropped by nearly a third, when roughly a gram of the compound was added to an animal's feed.
Then diluted solution of each compound was added.
Each compound was added to the perfusion solution in appropriate amounts immediately before the experiments.
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