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The signal of the compound was monitored at 254 nm for GA3.
The flow-rate was maintained at 1 mL/min and the fluorescence intensity of the eluting compound was monitored at emission and excitation wavelengths of 463 and 367 nm, respectively.
It should be noted that the nanostructure is designed to allow proper infiltration of the peroxidase enzyme (approximate size of 40 KDa), characterized by an average diameter of 60 to 80 Å, considering a globular conformation The functionalization of each compound was monitored through shift in reflectance peak.
The disappearance of test compound was monitored at 0, 5, 15 30, 45 min time points.
Each target compound was monitored in the selective ion monitoring mode (SIM) according to their retention time, using one quantitative ion (QT) and one qualitative ion (QL).
This compound was monitored by TLC and was detected by heating the plate at 110°C after spraying with p-anisaldehyde-sulfuric acid; furthermore, spectral results were in agreement with the published data [ 33].
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The functional groups present in native and parent compound were monitored by Fourier transform-infrared spectroscopy.
During enzymatic hydrolysis and fermentation, sugars, ethanol, and degradation compound were monitored using HPLC equipped with a refractive index (RI) detector (Waters 2414, USA).
MACCS consists of a set of 166 defined structural fragments whose presence or absence in a compound is monitored and ECFP4 is a topological fingerprint that generates varying numbers of atom environments for test compounds.
Removal of a group of secondary contaminants at the site, termed Varsol™ compounds, was monitored via concentrations of three Varsol™ marker compounds: decane, undecane and 1,3,5-trimethylbenzene.
The degradation rate of the organic compounds was monitored along the time in discontinuous experiments by analyzing the dissolved organic carbon concentration (DOC) in the solutions.
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