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This compound was initially solubilized in 98% ethanol, sterilized using 0.2 μm filters, added to 45°C YEM medium, and homogenized to obtain a final concentration of 0,2 g.l-1 of PCNB into the P-YEM medium.
The fourth sentence in the section Formulation and evaluation of the selective medium "This compound was initially solubilized in 98% ethanol, sterilized using 0.2 μm filters, added to 45°C YEM medium, and homogenized to obtain a final concentration of 0,2 g.l-1 of PCNB into the P-YEM medium".
The first reported such compound was initially characterized as ·nH2O in 1968, but was suggested in 1973 to actually have the formula ·2H2O based on the fact that Np VII) occurs as in aqueous solution.
Each compound was initially docked and then ranked by the predicted binding energy to obtain the line values in the network.
However, this compound was initially identified in a phenotype-based reporter-gene assay and, although additional studies have indicated TTSS specificity, the target protein is still unknown [39].
This compound was initially designed to compensate for a deficit in the natural anticoagulant protein C during sepsis, and thus it limited organ failures and improved the survival of septic shock patients [ 96].
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Each compound is initially assayed at 10 µM against each receptor, transporter or ion channel (primary assay).
Thus, according to KEGG nomenclature, the labels Phosphorylation, Dephosphorylation, Indirect, Expression and Compound are initially considered activations, unless the contrary is specified.
The LOPAC1280 library, comprising 1266 compounds, was initially screened in duplicate at 100 μ m yielding 170 active compounds with inhibition >50%% for TryR.
This library of 144 compounds was initially screened for inhibition of Mcm2-7, Mcm467 and TAg helicase activity at a final concentration of 1 mM (see Supplementary Table S1 available at http://www.bioscirep.org/bsr/033/bsr033e072add.htm) for chemical structures and a complete list of results.
Compounds were initially standardized using Chemaxon Standardizer [40], to ensure a consistent representation of compounds.
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