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The studied compound was immediately added at varying concentrations to an additional 100 μL of culture medium to obtain a total volume of 200 μL/well.
This latter compound was immediately quenched by adding it to a reaction mixture containing 3 a, the catalyst, and the base.
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This compound is immediately converted by CCD8 to carlactone, through a series of different reactions [ 33].
On the other hand, immunochemical detections such as ELISA have a fast turnaround, there is no need for any extensive sample preparation, and the compound is immediately identified.
The studied compounds were immediately added in varying concentrations in an additional 100 μL of culture medium to obtain a total volume of 200 μL/well.
Compounds were immediately added to a final concentration of 5 µM (LOPAC1280) or 1 µM (Prestwick, Spectrum).
Cell-based assays for drug-like molecules that inhibit Aβ42 aggregation are advantageous because toxic compounds are immediately discarded (Bharadwaj et al., 2010; Caine et al., 2007; Kim et al., 2006; Lee et al., 2009; Macreadie et al., 2008).
Its small concentration in wastewater, below 0.003 mM, and in the cell biomass (below 0.06 mM) is evidence that this compound was used immediately by the bacteria for energetic processes and metabolized fully.
The Mariners had seemed on course to avoid the play-off route until a last-minute equaliser by Northampton, which was immediately compounded by Leyton Orient's late winner at Oxford.
"The Israeli army announced officially that any international trying to leave the compound would be immediately deported and arrested, if not shot at," says Caoimhe. "By day three, it became glaringly obvious that I had made a huge mistake.
Biopsy material was immediately flash frozen and embedded in OCT compound.
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CEO of Professional Science Editing for Scientists @ prosciediting.com