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Each test compound was diluted in DMSO to 10 mM stocks, and added to the cultured embryos at a final concentration of 10 µM.
A clear reduction in the SRT was found in two-week old bees immediately after an odorant compound was diluted in the sucrose solution offered, in spite of the fact that the presence of odor (diluted in the food) had no impact on the energetic value of the circulating sucrose solution.
Each compound was diluted before use.
Each chemical compound was diluted into a concentration corresponding to the GARD input concentration.
Fifty milligrams of the tested compound was diluted in 2 ml of chloroform, homogenized and filtered.
The antibiotics used in combination were serially diluted along the ordinate, while the compound was diluted along the abscissa.
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And if a compound is diluted with a less toxic compound, the resulting mixture is assumedly not more toxic than the original compound.
Stock solutions of each compound were diluted with medium to the desired concentrations for specific experiments.
The compound is diluted to a final concentration of 10 mM in DMSO (cell culture tested, Sigma Aldrich), and for in vivo and in vitro assays diluted to the final concentration in complete RPMI-1640 medium.
Stocks of a standard mixture containing all eight vitamin E homologs (200 μg/mL for each compound) were diluted in hexane to generate standard curves for the analysis of the vitamin E homologs.
Twenty μl of plant sample or gallic acid (standard phenolic compound) were diluted with 1580 μl of distilled water and then mixed with 100 μl of 2 N Folin-Ciocalteu reagent.
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