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In separate experiment, cells were pre-treated with baicalein for 5 hours prior to infection and the compound was continuously present in the cell culture for 5 days until termination of the experiment.
After baseline values had been recorded at 1 Hz, solvent or one of the testing compounds at four concentrations (0.01 10 μ M or 0.1 100 μ M) (n=5 7 for each compound) was continuously superfused for 15 min for each concentration at a stimulation rate of 1 Hz.
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SSDSs are useful in cases where one specific compound is continuously used.
Under conditions where a compound is continuously present, there is an increased chance that toxic effects will have prevalence over realistic biological activity.
The authors suggested that drug development can be viewed as a model-building exercise, during which knowledge about a new compound is continuously updated and used to inform decision making and optimize drug development strategy.
Highly concentrated fraction of oxygenated compounds was continuously obtained for the desorption step and the blowdown step (depressurization).
Over the years, always new doping substances are synthesized and, as a consequence, the list of prohibited compounds is continuously updated and new suitable analytical methods for their detection and determination in biological matrices are continuously required.
Additionally, PM2.5, PM10, CO, nitrogen dioxide (NO2) and select volatile organic compounds were continuously measured at a downtown, third-story monitoring station along a busy roadway for 11 days.
Due to their various biological activities, the plant and its compounds are continuously under investigation.
The stimulus current required to evoke a 40% amplitude C-fibre compound action potential was continuously adjusted by the QTRAC software.
The white-coloured precursor was acidified using 0.1 M HCl, and in parallel, the resultant compound was stirred continuously at a stirring rate of 300 rpm.
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