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Moreover, supramolecular assembly of the title compound was analyzed by density functional theory (DFT) calculations.
The purified compound was analyzed on a nuclear magnetic resonance (NMR) spectrometer (JNM-ECP 400; JEOL, Tokyo, Japan), operating at 500 and 100 MHz for 1H and 13C, respectively, using methanol-d (CD3OD).
The rheological behavior and short-term aging of regenerated SBS modified asphalt binder was investigated and chemical compound was analyzed using Thin-layer chromatography/flame ionization detection (TLC/FID) and Fourier Transform Infrared (FTIR) Spectroscopy.
Based on these results, the compounds with antibacterial activity were screened by RT-PCR to determine whether they can regulate the expression of genes related to metabolism, haemolysis, and β-lactamase in vitro, and the structure microbicidal activity relationship of each compound was analyzed.
The labeled compound was analyzed by HPLC using a TSKgel Super-ODS column (4.6 mm inner diameter ×100 mm length; Tosoh Co., Ltd., Tokyo, Japan); the mobile phase was CH3CN/50 mM CH3COONH4 (35/65, v/v), the flow rate was 1.0 ml/min, and the retention time was 5.1 min for [11C]A-582941.
In further steps each compound was analyzed for its possible application as a drug.
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Tetramethylsilane is an inert liquid added in small amounts to the compound being analyzed.
The solid deposits formed from the model compound were analyzed by TEM to determine their internal structure.
The carbon and hydrogen content in the intercalation compound were analyzed on a CHNS-932 (LECO Instruments, Michigan, USA).
Samples coated with a matrix compound were analyzed by a MALDI LTQ Orbitrap XL mass spectrometer at a resolving power of 30 μm, spatial resolution.
To proof this prediction, HaCaT cells treated with each compound were analyzed for the induction of pro-inflammatory cytokines (IL1-β, TNF-α, IL-8 and IL-6).
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