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After compound treatment, cell viability was detected, and the cell growth curve was graphed.
After compound treatment, cell viability was detected and a cell growth curve was graphed.
Compound treatment was conducted once the myoblasts differentiated to form myotubes.
After compound treatment, cell viability was detected and cell growth curve was graphed.
Selective knockdown of Myc levels in these cells by RNA interference increased sensitivity to apoptosis with compound treatment.
This study provided the first evidence that compound treatment could induce a prominent step of salamander limb regeneration (cellularization) in mammalian muscle tissue.
Each compound treatment resulted in a unique gene expression signature of regulated genes.
Transcripts that are up/down regulated by either compound treatment (wotgfup/wotgfdown) or by TGF-β stimulation together with compound treatment (tgfup/ tgfdown) were detected based on all pair wise comparisons.
Hence the C2C12 hE50-GFP cenabledabled monitoring of both exon skipping (GFP) and mitotic index status (propidium iodide staining) in the same sample following compound treatment.
Genes were considered once the regulation is observed during compound treatment upon TGF-β stimulation as well as without TGF-β stimulation.
Neither protein showed a change in mass after compound treatment (Figure S1) indicating the compound did not bind covalently to either protein.
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