Exact(20)
After cryoprotection with 30% sucrose-PBS, tissue samples were embedded in OCT compound (Tissue Tek).
After dissection, kidneys were washed with phosphate-buffered saline and immediately frozen in tissue molds containing OCT compound (Tissue Tek).
Tissue was embedded in OCT compound (Tissue- Tek, Sakura) and snap-frozen in isopentane (Sigma-Aldrich, Inc).
Intact hearts were frozen in OCT embedding compound (Tissue Tek, Torrance, CA), and 10 µm cryosections were prepared.
After cryoprotection with 30% sucrose-PBS tissue samples were embedded in OCT compound (Tissue Tek, Sakura, The Netherlands).
After being frozen in OCT compound (Tissue Tek) in dry ice/n-hexane, the heads were cut into 10 µm sections using a cryostat microtome.
Similar(40)
Tumour tissues were embedded in Optical Cutting Temperature compound (Tissue-Tek, Sakura Finetek).
Following decapitation, dissected kidneys were quickly immersed in OCT compound (Tissue-Tek), frozen on dry ice, and stored at −80 °C.
Cryo-protected eye tissues were frozen in cryo-molds in O.C.T. Compound (Tissue-Tek; Sakura Finetek).
Each heart was removed, embedded in O.C.T.™ Compound (Tissue-Tek®; Zoeterwoude, Niederlande) and snap-frozen in liquid nitrogen.
Samples collected at post-mortem were snap frozen in O.C.T. compound (Tissue-Tek) and stored at −80°C until processing.
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