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72 available phenolic compound standards from diverse families present in fruits have been studied in order to analyze their fragmentation pattern.
1,1-Diphenyl-2-picrylhydrazyl (DPPH) and (3- 2-pyridyl -5,6,diphenyl-1,2,4-triazine-p, p′-disulfonic acid monosodium salt hydrate were from Merck, and Fe2+ chloride tetrahydrate ferrozine, and furanocoumarin compound standards, including xanthotoxin, bergapten, oxypeucedanin, imperatorin, phellopterin and isoimperatorin were from Merck.
Using these compound standards and published fragmentation data we analyzed total urine of BALB/cJ, C57BL/6J and CD-1 strains by LC- and GCMS for these previously identified pheromones.
See Table 2 for the breakdown seen from compound standards.
Phenolic compound standards were purchased from Sigma Aldrich (Milan, Italy).
This resulted in three sample groups with spiked-in compound standards.
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A TCh (as thiol compound) standard stock solution was prepared in the phosphate buffer (0.1 M and pH 7.4).
The exact mass to charge ratio (m/ z) of the molecules was extracted from the raw data or total ion chromatogram generating an EIC; and the retention time and accurate mass of each compound (standard and internal standard) were obtained.
Graph plotted between tested compound and standard drug presented in Fig. 4.
The EPA has set compound-specific standards for only seven of the hundreds of air toxics: asbestos, benzene, beryllium, inorganic arsenic, mercury, radionuclides and vinyl chloride.
Multiple reaction monitoring was used for the final comparison of unknown compounds with standards.
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