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Utilizing additional fragment collections, we expanded our previous ~2,500 compound screen of the MAP4K4 kinase [27] by performing an additional screen of 8,000 compounds [28].
Here, we review the principles of high-content screening technology and summarize efforts to carry out small compound screen using podocytes.
Braungart et al. [54] performed a focused compound screen using the C. elegans MPTP model of PD and found that lisuride and apomorphine (dopamine receptor agonists), as well as rottlerin (protein kinase C inhibitor) ameliorated the MPTP-induced behavioural defects when present at a low concentration.
Equation (1) is then fitted to the dose response curve of each single compound screen.
The DSF compound screen generally followed the protocol published by Niesen et al. [11].
Compounds that may fall into this class are often not identified in a traditional antifungal compound screen as the screen may rely solely on inhibiting microbial growth.
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X. Ding and G. Tang contributed to compound screening.
Results of the secondary compound screening are shown in Table 2.
The desired output in initial compound screening is an IC50 value.
In this review, we focus on compound screens performed in the nematode worm, Caenorhabditis elegans.
NCGC's Inglese has compound screening experience in both biotech and pharmaceutical companies.
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CEO of Professional Science Editing for Scientists @ prosciediting.com