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The lower panel of Figure 1 shows the optimized MS/MS compound parameters (left) and ion source parameters (right).
The optimized compound parameters for monitoring LHA were set as follows: DP 48 V, EP 5 V, CE 20 V, and CXP 8 V.
The compound parameters like cone voltage and collision energy were set at 46 V & 30 eV for linifanib and 36 V & 22 eV for IS respectively.
The optimized compound parameters for monitoring LS and IS were set as follows: declustering potential (DP), 38 V; entrance potential (EP), 10 V; collision energy (CE), 20 V; and collision cell exit potential (CXP), 7 V.
At the end, the highest peak area for molecular ion of the analyte was achieved when the optimized compound parameters and ion source parameters were combined (Figure 2). Figure 2 MS/MS parameters and LC conditions optimization.
The MS analyzer parameters were as follows: LM1 and HM1 resolution 14.0 and 14 respectively; ion energy 1; LM2 and HM2 resolution 10.0 and 14.0 respectively, ion energy 2. The compound parameters like cone voltage and collision energy were optimized and set at 27 V and 19 eV for LND and 36 V and 36 eV for IS respectively.
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The sub-watershed with the least compound parameter value was assigned as highest priority.
Finally, the highest score of compound parameter was assigned last rank (Fig. 4).
The sub-watershed with the lowest compound parameter value was given the highest priority.
The compound parameter values are calculated and the basin with lowest compound weight has been given highest priority.
After analysis of morphometric parameters, compound parameter values are calculated and prioritization rating of 14 sub-watersheds is carried out.
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Since I tried Ludwig back in 2017, I have been constantly using it in both editing and translation. Ever since, I suggest it to my translators at ProSciEditing.

Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com