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The sub-watershed with the least compound parameter value was assigned as highest priority.
Finally, the highest score of compound parameter was assigned last rank (Fig. 4).
The sub-watershed with the lowest compound parameter value was given the highest priority.
Sub-watershed 8 with a compound parameter value of 3.57 receives the highest priority (one) with next in the priority list is sub-watershed 7 having the compound parameter value of 3.64.
The sub-watershed 13 with a compound parameter value of 3.63 received the highest priority (one) with the next in priority being sub-watershed 11, having a compound parameter value of 4.25.
Here sub-watershed 8 with a compound parameter value of 3.00 (minimum) receives the highest priority (one) and next sub watershed 7 having the compound parameter value of 3.33 receives the next priority (two).
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The lower panel of Figure 1 shows the optimized MS/MS compound parameters (left) and ion source parameters (right).
The optimized compound parameters for monitoring LHA were set as follows: DP 48 V, EP 5 V, CE 20 V, and CXP 8 V.
The compound parameters like cone voltage and collision energy were set at 46 V & 30 eV for linifanib and 36 V & 22 eV for IS respectively.
At the end, the highest peak area for molecular ion of the analyte was achieved when the optimized compound parameters and ion source parameters were combined (Figure 2). Figure 2 MS/MS parameters and LC conditions optimization.
The optimized compound parameters for monitoring LS and IS were set as follows: declustering potential (DP), 38 V; entrance potential (EP), 10 V; collision energy (CE), 20 V; and collision cell exit potential (CXP), 7 V.
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