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In this process, cells capable of producing tissue interact with chemotactic factors to form bone. Firstly, osteoblasts secrete substances to form osteoid tissue or immature bone, a nonmineral matrix compound of collagen [ 168] and glycosaminoglycans.
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This inhibitory effect clearly indicated the effect of test compounds on collagen receptors i.e. GP-IIb/IIIa or VI [24].
We next tested the effect of the inhibitory compounds in the presence of collagen, a more physiological platelet agonist than arachidonic acid.
Results represent the aggregation curves of collagen with inhibitory compounds of type I and ibuprofen (A) and compounds of type II (B).
Stripping an organ leaves a natural scaffold of collagen and other compounds, called the extracellular matrix, which provides a framework for new cells and preserves the intricate internal architecture of the kidney as well as its basic shape.
For this purpose, however, further investigations are needed to determine the compound's effects on the expression and release of collagen and other extracellular matrix components.
The relevance between the therapeutic effect and effective compounds focused on immunoregulation, anti-inflammation, the inhibition of collagen formation and the regulation of muscle contraction.
These compounds are likely associated with intra- and intermolecular cross-linking of collagen as the intensity of the color corresponded well with the collagen stability against enzymatic digestion.
Thus, these two newly formed reducible compounds are likely associated with GE-induced cross-links involving Lys and Hyl residues of collagen.
The degradation of collagen, as well as of a great number of other extracellular matrix compounds, is initiated by metalloproteinases (MMPs).
The expression profile observed in gills confirmed previous studies showing that collagen is a major compound of this tissue [ 67].
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