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The minus pattern (lines 17 21) eliminates all identified compounds (to which COMPOUND is bound) that are annotated as antibiotics.
Suppression of microbial metabolites in the in vitro colon model can be caused by enzymatic inhibition, when the inhibiting compound is bound to the active site of the enzyme and its substrate cannot be attached to it.
The only elements of the model interfering with the predictability of the combined oscillation are: 1) the stochasticity surrounding when each compound is bound and rebound and 2) that some compounds are metabolized and their product moved into the BileCanal, and no longer contribute to the profile.
These results indicate that a maximum of 1 mol of compound is bound per mole of DNA sites at saturation. Figure S2 of the Supporting Information shows representative Tm plots of selected compounds in the presence of the 5′-ATGA-3′ sequence, and Tm values are listed in Table S1 of the Supporting Information.
Because all the ligands bind quite strongly to the sequences in this work, the heat/mol of added compound is essentially constant in the initial titration region where all added compound is bound to DNA.
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DOI: http://dx.doi.org/10.7554/eLife.07777.013 These results led us to re-examine the finding in Figure 4A, where the printed lead compound was bound by a bacterially expressed recombinant GST fusion protein (which likely contained co-purifying contaminating nucleic acids).
The binding of label compounds to DNA, RNA, and protein generally reflected the distribution data, thus more compound was bound in the stomach, liver, and lung after oral administration compared to topical application, whereas the opposite was true for the skin.
This dominance of the electrostatic energy is in contrast to the case that low molecular-weight compounds are bound to their targets.
Ribosylated compounds are bound reversibly and specifically at the 2',3'-cis-diol group.
Soluble compounds are mainly located in the vacuoles whereas insoluble compounds are bound to the cell walls [ 42].
Structural examination of the area where compounds are bound to the PPARγ receptor yielded evidence that TBBPA, TCBPA, and other halogenated BPA derivatives partially activate this receptor.
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