Following resolution, the stain-free compound was activated (Posch et al. 2013) and proteins were transferred to an Immobilon FL PVDF membrane (Millipore, no. IPFL00010) at 100 V for 60 min. Total protein, pre- and post-transfer, was monitored using the stain-free fluorophore as described (Posch et al. 2013).
These compounds are activated intracellularly via the same metabolic pathway as ara-C.
Such compounds are activated by the enzyme to the corresponding radicals.
Previous studies have shown that genes responsible for controlling the glyoxylate cycle and gluconeogenesis, which are used for the assimilation of two carbon compounds, are activated after C. albicans is exposed to macrophages.
Photodynamic therapy, in which systemically administered photosensitive compounds are activated by an external light source, can be considered a related strategy to drug delivery and is under investigation for the treatment of superficial breast tumors.
Here, we describe CH-01, which is a proof-of-concept compound that we propose is activated as a Chk1 and Aurora kinase A inhibitor after the hypoxia-promoted loss of the 4-nitrobenzyl group.
Such data would indicate whether the NADPH oxidase-activating compound phytol is activating a pathway that is normally inactive in the absence of a fully functional Ncf1.
In order to rule out the possibility that amphiER is activated by a compound related to E2, a large panel of 14 other steroids and cholesterol derivatives were tested for their ability to bind and activate amphiER.
After the synthesis of gentisate, this compound will be activated by the addition of a CoA group.
Our finding that some of the mutagenic activity of one sample (B1) from the brominated pool water was due to activation by GSTT1 suggests the presence of compounds that are activated to mutagens by this enzyme, such as brominated THMs (DeMarini et al. 1997; Pegram et al. 1997), some methylene dihalides and bifunctional butanes (Thier et al. 1995), and/or 1,1-dichloropropene (Granville et al. 2005).
