However, how to determine which compound combinations exhibit a desired form of synergy in a particular case is by no means clear, and the effect of multiple bioactive compounds in parallel is overall rather poorly understood.
We designed a simple ELISA-based HO-1 enzyme activity assay, which allows for the screening of 12 compounds in parallel in the setting of a 96-well plate.
In all cases where we apply tests to multiple compounds in parallel, we use the Benjamini-Hochberg False Discovery Rate (FDR) [32] at a value of q = 1/25.
GC-MS experiments on complex biological and environmental samples may result in hundreds of signals and the detection of many compounds in parallel.
Therefore, there is great enthusiasm to develop a preclinical system that would allow for high-throughput testing of cancer cell lines against large numbers of drug compounds in parallel.
Their voltammograms were compared with that of 1,2-dihydroxybenzene (catechol), 1,3-dihydroxybenzene (resorcinol) and 1,4-dihydroxybenzene (hydroquinone), which were measured as control compounds in parallel to that of D1 and D3 (Nasr et al., 2005; Astudillo et al., 2007; Nematollahi and Mohammadi-Behzad, 2009).
For comparison, the effects of another compound NS1643 was in-parallel assessed, which also acts primarily to attenuate channel inactivation with moderate potency.
The LOX inhibitory activity of the compound 14e proceeds in parallel to the % inhibitory value of lipid peroxidation meaning that this LOX inhibitory activity is supported by the lipid peroxidation inhibition.
The fractional inhibitory concentration (FIC) was calculated using the MIC from the checkerboard assay and the MIC of each compound alone, obtained in parallel in the same assay, according to the following formula: FIC = MIC of antimicrobial agent in combination/MIC of antimicrobial agent alone.
In this study, we used a panel of 36 human cancer cell lines, flow cytometry and oligonucleotide microarrays to analyze further these two compounds in a parallel fashion with regard to both antitumor activity (phenotype) and gene expression (genotype) bases.
