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Cyclic di-GMP capture compound experiments were performed as described previously (Nesper et al., 2012) with minor modifications.
Cyclic di-GMP capture compound experiments were performed as described previously (Nesper et al., 2012) but with the minor modifications described in Extended Experimental Procedures.
c-di-GMP Protein Capture Experiments Cyclic di-GMP capture compound experiments were performed as described previously (Nesper et al., 2012 ) with minor modifications.
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Drawing on a review of the chemical and physical properties of these two organic compounds, experiments were performed using high-pressure differential scanning calorimetry (DSC) and a batch reactor.
All the experimental results show that the foam agents have good effects of profile control and incremental oil in high salinity reservoirs, and the compounded experiment is an effective way to improve properties of the foam system.
Compound exposure experiments were performed in the dark at 28.5°C.
Compound exposure experiments were performed on wild-type larvae from 2 days post-fertilization (d.p.f).
To ensure that the effect of MβCD on C. jejuni internalization was not unique to this chemical compound, similar experiments were performed with 2-hydroxypropyl-β-cyclodextrin (HPβCD).
After identification of adequate concentrations viability and MINC data were obtained for each compound, and experiments were repeated at this test concentration with two further cell preparations (Figs. 5, 6).
Compound exposure experiments were performed on wild-type larvae (TL strain) from 2 to 3 days post-fertilization (d.p.f .. Concentration response assays were performed over log intervals, namely from 1 n m to 100 μ m for PPP, in order to determine the maximum non-toxic concentration (MTC) for subsequent autophagy assay experiments (n = 30 larvae per concentration).
Independently of the gas used and in order to improve reproducibility of the experiments, as well as to standardize any possible effect on the loss of volatile compounds, successive experiments were performed using a programmed on/off pattern for the gas valve, mimicking the pattern obtained in that preliminary experiment (Table S1 in the Supplementary Material).
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Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com