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The spiro-compounds were evaluated as acetylcholinesterase and butyrylcholinesterase inhibitors (AChEIs/BuChEIs) respectively, and the most potent compound exhibited a moderate AChE inhibitory activity (5f: IC50 = 84 μM).
This compound exhibited a binding affinity value of 0.094 nM toward human 5-HT4R and a high selectivity over other serotonin receptor subtypes (5-HTR).
The spinel compound exhibited a slightly smaller lattice constant than a conventional spinel compound, even though the cationic ratio of Li/Mn is the same for both compounds.
The compound exhibited a reversible discharge charge behavior between 0 and 0.75 V versus Li/Li+ with a first discharge capacity of 293 mA h g−1.
Lipophilicity of this compound is in the low end of the desired range but it is interesting to note that the compound exhibited a ligand lipophilicity efficiency (LLE) >5 based on ClogP and almost sustained potency on both mSUCNR1 and hSUCNR1.
The human cancer cell lines SMMC-7721 (liver cancer) and SK-OV-3 (ovary cancer) were tested for their viability by the MTT method in vitro, which showed that the compound exhibited a remarkable activity against two cell lines with IC50 values smaller than 0.24 μg/mL, 0.32 μg/mL, respectively.
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The most potent compound exhibited an IC50 value lower than 20 μM in an in vitro enzymatic assay.
A compound was considered toxic, when the absorbance of the cells exposed to the compound exhibited an absorbance lower than the control's absorbance (Gallego et al. 2006).
The compound exhibits a random distribution of the metal ions in octahedral 4a positions.
At a current density of 1600 mA g−1, the compound exhibits a stable discharge capacity of about 556 mAh g−1.
It is observed that the Mg-free compound exhibits a much lower reversible hydrogen capacity than the Mg-containing one.
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