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Exact(11)
In the control strain TMB4444, only glycogen was detected as carbon storage compound (data not shown), whereas PHB and glycogen were found in TMB4425 and TMB4443.
Eventually, conidia could form small conglomerates, which grew laxly, along with hyphae originated from single, i.e., non associated conidia, apparently free of extracellular compound (data not shown).
Both 4-PPB and 4-APB showed approximately the same concentration-dependent inhibition of parasite invasion and rhoptry secretion as 4-BPB, indicating that the 4-bromo is not required for the activity of the compound (data not shown).
hCNT2 transported neither compound (data not shown).
Similar results were obtained for an in-house substrate-selective compound (data not shown).
Similar effects were obtained with the CCX771 compound (data not shown) that was used in the following experiments.
Similar(49)
The regional differences in radioactivity were abolished by the pretreatment with 10 mg/kg of thioperamide, an H3R antagonist (Figs. 3d f, 4d f), or 1 mg/kg of unlabeled corresponding compounds (data not shown).
We observed micromolar potencies for both compounds (data not shown), similar to those observed in the EFC assay (Figure 1A).
In the initial screen, we used a chemical library containing 206 highly selective compounds (data not shown), most of which had been previously shown to exhibit the anti-fungal activity by inhibiting sterol or chitin biosynthesis [15], [16].
NYH/CHS and NYH/APO866 displayed similar resistance to the compounds (data not shown).
Their biointermediates in the anoxic reactor consist of at least three known compounds (data not shown).
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