Sentence examples for compound concentrations added from inspiring English sources
The compound concentrations added to the water were higher than those necessary in the culture medium to obtain a response, likely reflecting systemic mechanisms such as uptake, metabolism, and excretion dynamics of the compounds.
Varying compound concentrations in 10 µl of DMSO were added to 490 µl of diluted MTs at 37°C.
The affinity of several millimolar is sufficient to detect binding by X-ray crystallography if the added compound concentration is of the order of 1 mM.
Various concentrations of compounds were added to obtain final concentrations of 0.5, 5, 50 μg/mL, each in triplicate.
Initial concentration of compound A added to the reactor can be measured by PTR-MS and confirmed by calculations based on the volume of compound A added, VA.
Assay mixture (180 μL) containing three different concentrations of test compound was added to three rows of a microtitre plate, a fourth row contained only the assay mixture.
The medium was then changed to DMEM/Ham's F-12 medium containing 2.5% charcoal-stripped FBS, and either vehicle [dimethyl sulfoxide (DMSO)] or different concentrations of the compound were added.
Aliquots of the assay mixture (180 μL) containing three different concentrations of test compound were added to three rows of a microtitre plate, a fourth row contained only the assay mixture.
The half maximal inhibitory concentration of each compound was added to the reactions (4 m - 197.3 μM; 4bt (known as dimethylenastron) - 126.9 μM; 4p - 87.54 μM; 4bc - 234.9 μM; 4x - 276.5 μM and monastrol – 110.4 μM) with readings taken at 30-second intervals for 30 minutes at room temperature using a spectrophotometer at 360 nm.
For these assays, vehicle or test compound (2× final concentration) was added, followed by varying concentrations of glutamate 2.5 min later using FDSS 6000.
For this purpose, MTs were assembled from 10 µmol/l αβ-tubulin; 5 min into the reaction compound B was added in concentrations up to 200 µmol/l.
