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Microsomal stability was studied in mouse liver microsomes (0.5 mg/mL) at a final test compound concentration of 1 μM and performed in triplicates in accordance to the published protocol22.
Approximately 25 mg of the rSP-C33Leu dry peptide was dissolved in a CDCl3/CD3OD 33:66 (v/v) solution to obtain a final compound concentration of ∼1.7 mM.
Worse, the ITC experiment only achieves a compound concentration of about 17% of the saturated concentration in the syringe (see Figure 6).
Using a compound concentration of 25 µM and an exposure time of 24 hours, 20 compounds were identified as inducing apoptosis above a preselected threshold value (Table 1).
The HTS Assay was performed at a final compound concentration of 10 µM (1% DMSO), with cell densities of 2×105 cells/ml (2×105 cells) in a volume of 40 µl.
For the primary screen, the compounds satisfying the criterion of z-score >2 or <−2 were subjected to a secondary screen at an increased compound concentration of 200 uM (10∶1 molar ratio to heme).
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Synthesis of fewer than a dozen compounds was required to achieve inhibition of the growth of methicillin-resistant Staphyloccus aureus (MRSA) at compound concentrations of 1.56 μM.
Fig. 2 Example of simultaneous multi-element HPLC-ICP-MS analysis of a mixture of different compounds used in imaging applications at approximate compound concentrations of 1 μM.
For biodynamic model validation it is very important that the difference between the values of the parameters obtained theoretically and experimentally were near to zero. Figure 3 Comparison of simulated and experimental key compound concentrations of B. thuringiensis var.
Unfortunately, establishing synergistic compound combinations at greater scale is typically hampered by the necessity to screen multiple compound concentrations of one compound against different concentrations of another compound.
The kinase and phosphatase libraries were screened in 96-well format with compound concentrations of 100, 10, 1 µm and 100 nm.
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