Exact(7)
The considerations for the construction of the model were: a) each phase of B. thuringiensis culture can be represented by its principal metabolic pathways (EMP for vegetative growth, GABA for transition and TCA for sporulation); and b) each pathway can be follow with its key compound (cell for EMP, PHB for GABA and DPA-Cry protein for TCA).
To exclude toxic effects of the compound, cell viability was determined by trypan blue (Gibco/Invitrogen).
Because this was assumed to reflect compound cell stress of the transfection and IL-1β treatment, this was not further evaluated here.
Compound cell stresses inherent in biomechanical injury may have accounted for this distinction in UPR response, relative to the response to IL-1β in isolation.
Gymnemic acid being an important bioactive compound, cell suspension cultures of G. sylvestre were generated and optimized for the production of gymnemic acids [ 103, 104].
Interestingly, genes showing HSB biased expression are associated with synaptic transmission, response to gonadotropin and purine-containing compound, cell adhesion, and transmembrane transporter activity (Additional file 4: Table S3).
Similar(53)
Thus, module 1 and module 2 are related with module 3. Phase: Vegetative growth; metabolic pathway: Embded-Meyerhof-Parnas pathway; key compound: cells.
After 72 hours treatment with 0.3 μM, 1 μM and 3 μM azacitidine compound, cells were trypsinised and counted.
Thirty six hours after the addition of test compounds, cell viability was measured by the MTS assay.
Several GO terms were highlighted with significant enrichment, including production of defense-related compounds, cell wall components, cell signaling proteins, and transcription factors.
After exposure to the compounds, cell growth was determined by measuring the formazan formation from 3- 4,5-dimethylthiazol-2yl -2,5-diphenyltetrazolium 3- 4,5-dimethylthiazol-2yl -2,5-diphenyltetrazolium 3- 4,5-dimethylthiazol-2yl -2,5-diphenyltetrazolium 3- 4,5-dimethylthiazol-2yl -2,5-diphenyltetrazolium 3- 4,5-dimethylthiazol-2yl -2,5-diphenyltetrazolium 3- 4,5-dimethylthiazol-2yl -2,5-diphenyltetrazolium
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