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In soil simulation tests, soil samples are incubated with the test compound and analyzed for the parent compound and transformation products after appropriate time intervals.
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We synthesized a series of vanillin-derived compounds and analyzed them in HeLa cells for their effects on the proliferation of cancer cells.
First, we plotted the Docking Scores (DS) of these compounds and analyzed their variations based on the type of co-binding peptides.
We synthesized these compounds and analyzed their activities.
Then we removed the redundant compounds, and analyzed which compounds can target more than two proteins simultaneously.
The MOLM-13 cells were incubated with various concentrations of the compounds and analyzed by measuring the absorbance of formazan.
Uptake assays were always performed in the presence of the compounds and analyzed after 2 or 4 h by immunofluorescence microscopy or flow cytometry.
The medium was then changed to DMEM High Glucose containing 2.5% charcoal-stripped FBS and treated with either the vehicle (DMSO) or the indicated compounds and analyzed by western blots as described [ 20, 21].
As we had a high degree of correlation between many of the 23 PAHs measured (data not shown), we grouped these compounds and analyzed them as low-molecular-weight (MW; < 190) and high-MW (> 190) species associated with PM2.5 10 and PM2.5 10, respectively.
After 8 12 weeks, teratomas were excised, fixed, dehydrated, embedded in O.C.T compound, sectioned, and analyzed by immunostaining.
Among 15 compounds designed and analyzed, we have synthesized 5 compounds and evaluated their cytotoxicity against drug-sensitive and multidrug-resistant cancer cell lines.
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