Therefore, this study aimed to identify the best combination of the compost tea extraction parameters for exalting both chemical and microbiological features.
The total fungal RNA of the compost was extracted from 1 g of each compost sample, which was ground to fine powder in liquid nitrogen using a mortar and pestle, followed by the extraction and purification protocol for filamentous fungi using Qiagen RNeasy Plant Mini Kit QIAGEN USAA, Valencia, CA).
Aqueous extraction of compost, casing layer and fruiting bodies revealed that more than 95% of carbohydrates are insoluble.
Recent investigation of microbial communities in BP-degrading compost by using direct DNA extraction from compost followed by cloning and sequencing showed that Ascomycota comprised the most dominant group of microorganisms during the biodegradation of BP films (Sangwan and Wu [2008]; Sangwan et al. [2009]).
Each DNA sample for next-analysis was the mixture of three independent DNA extractions from one compost sample.
Results suggested that the best combination of the studied parameters was: 1 2.5 compost/water ratio, 2 days of extraction time and the compost tea should be utilized immediately after the extraction, since the storage reduced the microbial populations.
Moreover, BEs comprise non-living agricultural bio-stimulants (ABs) such as extraction products from macroalgae, compost, and plants [12].
A 100 ml of compost washing was taken in 500 ml extraction bottle and then added 200 ml of methanol.
Furthermore, as development of this work, the extraction and characterization of humic water-extractable fraction in composted samples could contribute to clarify these aspects.
Samples from 3, 6, 9, 15, 18, 24, and 27 weeks of composting were collected for total genomic DNA extraction.
