Sentence examples for composition of the reads from inspiring English sources

Nucleotide composition of the reads appears biased at their 5′end, with U being the first nucleotide on almost 60% of the reads.

The resulting running times and accuracies depend heavily on the exact composition of the read set with respect to the types of matches.

This model takes into account the ℓ-mer composition of the read and how similar it is to the set of ℓ-mers of reads that have already been placed in this bucket.

Overall, horse reads recovered from the DNA phosphatase treatment were found to be slightly shorter than reads recovered without DNA phosphatase treatment and the base composition of these reads was similar to those recovered following the use of a 95°C denaturation temperature.

We examined the lengths and nucleotide composition of these reads and found two characteristics similar to those of general nucleosome cores: First, a predominant length of ~155 bp, slightly larger than the 147 bp typical of canonical H3 nucleosome core DNA in other organisms.

A summary of the number of reads and the composition of reads generated from the RNA-seq data is shown in Table  2 while steps taken to assemble the transcriptome from clean reads are shown in Figure  1.

Furthermore, we used a simple set of rules to choose short sequence tags that balanced the nucleotide composition in the first 4 positions of the reads, that maximized the stagger in the primer sequences when attached to the solid surface and that minimized the possibility of primer-dimer formation.

The read classification based on sequence composition relies on the intrinsic features of the reads.

mRNA sequencing libraries have been suggested as a valid replacement for the control lane, however mRNA libraries (prepared from the standard Illumina-protocol using double strand ligation protocol) show a biased base composition in the first twelve bases of the reads [ 42] and are therefore no longer recommended.

This change in sequence composition suggests that a significant fraction of the reads consisted in oligonucleotide sequences that could not be filtered, possibly due to the presence of substantial levels of sequencing errors and/or the formation of chimeric sequencing reads.

Finally, variation in extraction techniques and/or sample preparation kits have been shown to affect the composition of sequence reads [ 8, 9].