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OPB-31121 did not significantly affect the number and composition of colonies (Supplementary Figure S3A).
Details on the origin and composition of colonies used in this study are given in Table 1.
Here, we examine the second hypothesis and consider the relationship between the phenotypic composition of colonies and the presence of foreign spider species.
To test for relationships between colony size, phenotypic composition of colonies, and the richness/abundance of foreign spiders, we used a general linear models (ANCOVAs) with the number of asocial colony members as our independent variable, colony size (i.e., the number of resident females) as a covariate, and web-associate abundance and genus richness as response variables.
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Consequently, our detection methods may fail to ascertain the complete lineage composition of all colonies, especially in small colonies.
Conversely, the formation and composition of mutant colonies were weak in presence of 10% ETOH compared to the low concentrations.
The size and composition of those colonies is unique to each of us, almost like a fingerprint.
The genetic composition of founder colonies was determined by microsatellite typing.
To assess the lineage composition of the colonies, cells were fixed on plates for 20 min in methanol, at -20°C, and were then stained using Peroxidase Histostain-Plus and Alkaline-phosphatase Histostain-Plus kits (Zymed, South San Francisco, CA, USA), according to the manufacturer's protocol.
The fault, in their view, lay with no single person, but with the middle-class composition of the colony, which, feeling itself imperiled, had acted instinctively, as an organism, to extrude the riffraff from its midst.
Thus, the genotype composition of this colony is consistent with monogyne social organization.
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