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Briefly, after 12-h fasting, all individuals underwent anthropometrical evaluation including body composition evaluated by bioelectrical impedance, and a 75-g OGTT was performed with 0-, 30-, 60-, 90-, and 120-min sampling for plasma glucose and insulin.
EDS data showed that the composition of the oxide films is quite different from the nominal composition evaluated from the precursor solutions.
Samples were analysed to determine dissolved organic carbon, nitrogen and phosphorus fractions with DOM composition evaluated via the DOC DON ratio, DOC DOP ratio, specific UV absorption at 254 nm, absorbance ratio (a250 a365) and the spectral slope parameter between 350 and 400 nm (S350 400).
At the finest scale of community composition evaluated (100 groups), the estimate ranged from 37% to57%.
Moreover, the response to diet composition evaluated through mRNA levels and fatty acid composition showed interesting differences between Alentejana and Barrosã bulls.
Blood sampling, autonomic balance evaluated by heart rate variability analysis, clinical assessment including vital signs, casual blood pressure, electrocardiogram (ECG), body composition evaluated by bioelectrical impedance measurements, abdominal circumference in three predefined points and anthropometric assessment by calliper were evaluated at predefined time points as indicated elsewhere.
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Details of all of the topological tree compositions evaluated in this study can be found in Figure 3 (Supplementary section).
Dietary coding utilised McCance and Widdowson "Composition of Foods" (6th edition) food codes and nutrient composition was evaluated using WISP 3.0 (Tinuviel Software) for GI and GL values [ 29].
Their chemical composition was evaluated by GC-MS.
Chemical composition was evaluated by microprobe analysis.
Silage chemical composition was evaluated only at 56 d.
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