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Kinases were diluted in buffers of different composition, depending on the kinase assay, consisting of one or more of the following chemicals: MOPS, EDTA, Brij-35, Glycerol, NaCl, β-mercaptoethanol, BSA, HEPES, Triton X-100, DTT, Triton Surfactant, Glycerol, TRIS, EGTA, Tween 20, Na-β-glycerophosphate and Na3VO4.
All cells contain enzymes, which usually vary in number and composition, depending on the cell type; an average mammalian cell, for example, is approximately one one-billionth (10−9) the size of a drop of water and generally contains about 3,000 enzymes.
Hence a clear molecular profile was evident dependent on disease status of the joint, which however changed in composition depending on the biological sample analysed.
There are three basic forms of composition depending on the type of task.
The fringe-shifts are converted to temperature, pressure, or composition depending on the configuration.
Honey is composed principally of fructose and glucose, the composition depending on the original nectar collected by the honeybee and on the amount of processing and storage time.
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Its composition depends on the manufacturing process and the chemistry of the original oilfield.
Its composition depends on the characteristic of the watercourse.
Plant composition depends on a balance between air, soil, and water.
Species composition depended on several topography, soil, and field management factors.
The results show that the optimal catalyst composition depends on operating cell voltages.
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