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To test for defects in cell wall composition, cells were grown overnight with or without DOX and treated with Zymolyase 100T (β-1,3-glucanase; Sunrise Products Inc., Waterville, MN) as described previously (27).
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In natural tissues, the extracellular matrix composition, cell density and physiological properties are often non-homogeneous.
The parameters investigated included solvent, pH, temperature, sonication, catholyte composition, cell design, flow rate, anode size and condition, monomer concentration and supporting electrolyte concentration.
Engineering interface tissues is a complex process, which requires a combination of specialized biomaterials with spatially organized material composition, cell types and signaling molecules.
These findings suggest that C. neoformans protein-O-mannosyltransferases play a crucial role in maintaining cell morphology, and that reduced protein-O-glycosylation leads to alterations in stress resistance, cell wall composition, cell integrity, and survival within the host.
Therefore, tendons from different sites have differences in their structure, composition, cell phenotypes, and metabolism [ 12].
LCPUFAs may influence immune cell function through a number of mechanisms related to membrane composition, cell signalling, and gene regulation, among others [ 2].
It might be argued that IHCs of HC-BK−/− mice are developmentally impaired with regard to their ion channel composition, cell size, exocytosis, or tonotopic specialization.
Microfabrication provides a useful and promising technology for the design and control at micrometer scale of cellular microenvironments including substrate topology and biochemical composition, cell types surrounding the cells of interest, and medium composition.
In contrast to humans, disc degeneration is not commonly seen in most quadrupedal animals, likely due to a variety of factors such as mineral composition, vertebral range of motion, extracellular matrix (ECM) composition, cell type, and weight distribution [ 7].
With optimized compositions, cell-covered dispersed oil droplets were stable against droplet coalescence and bulk phase separation for over four months.
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